What does MALS measure?
A multi angle light scattering (MALS) detector is a form of static light scattering detector which allows the absolute molecular weight (Mw) and potentially the radius of gyration (Rg) of a sample to be measured.
What does SEC-MALS tell you?
SEC-MALS determines molecular weight from 200 g/mol to 1 billion g/mol. It can also determine molecular size – the rms radius, or radius of gyration Rg – from 10 nm to 500 nm and beyond. By combining molar mass and size, it can also assess molecular conformation. And MALS detectors only require annual calibration.
How does multi angle scattering work?
Multiangle light scattering (MALS) describes a technique for measuring the light scattered by a sample into a plurality of angles. It is used for determining both the absolute molar mass and the average size of molecules in solution, by detecting how they scatter light.
How much protein do you need for SEC-MALS?
➢ For the Shodex protein columns 100 µl 1mg/ml protein is a good starting point. Small proteins (< 10kD) give lower light scatter signals and you might have to increase the protein concentration. ➢ Samples should be filtered through a 0.1 µm filter or centrifuged to remove precipitates and larger insoluble particles.
What is DN dc value?
What is dn/dc? The key parameter for translating RI detector output to exact sample concentration is the dn/dc value, or refractive index increment. This value is unique for a sample-solvent combination, as it represents the difference in refractive index between the sample and the solvent.
What is SLS and DLS?
The evaluation of the fluctuations is commonly named as dynamic light scattering (DLS) while the analysis of the absolute mean intensity is known as static light scattering (SLS). The intensity is very sensitive to variations in size of the solutes, so that it is advantageous to investiagte aggregation in solution.