What is BrdU assay?

What is BrdU assay?

Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.

How does BrdU labeling work?

Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. Once incorporated, these nucleoside analogs serve as cell cycle and proliferation markers that can be detected using labeled probes to identify cells that are actively proliferating.

What is the purpose of BrdU?

Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation.

Is BrdU toxic?

BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.

What does Ki67 positive mean?

One of the things that indicates a cell has mutated (been altered) into a cancer cell is that the cell does not stop growing and dividing. Because Ki-67 is associated with cells that are rapidly growing and dividing, it is sometimes considered a good marker of proliferation (rapid increase in the number of cells).

Is BrdU a carcinogen?

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light.

What is monoclonal anti-BrdU?

Monoclonal anti-BrdU (immunohistology grade) (mouse IgG1 isotype) is derived from the BU-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with bromodeoxyuridine conjugated to KLH. Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier.

What is the source of anti-BrdU?

BrdU solutions are sensitive to light. Monoclonal anti-BrdU (immunohistology grade) (mouse IgG1 isotype) is derived from the BU-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with bromodeoxyuridine conjugated to KLH.