What is Chimera detection?

What is Chimera detection?

Chimeras are artifact sequences formed by two or more biological sequences incorrectly joined together. This often occurs during PCR reactions using mixed templates (i.e., uncultured environmental samples).

What is chimeric PCR?

PCR chimera It occurs when the extension of an amplicon is aborted, and the aborted product functions as a primer in the next PCR cycle. The aborted product anneals to the wrong template and continues to extend, thereby synthesizing a single sequence sourced from two different templates.

What are chimeras sequencing?

Chimeras are sequences formed from two or more biological sequences joined together. Amplicons with chimeric sequences can form during PCR. Chimeras are rare with shotgun sequencing, but are common in amplicon sequencing when closely related sequences are amplified.

How do you get rid of a chimeric sequence?

When a sequence is flagged as chimeric in one sample, it can be removed from only that sample by setting dereplicate = true, or from all samples by setting dereplicate = false.

What does chimeric mean?

Listen to pronunciation. (ky-MEER-ik) Having parts of different origins. In medicine, refers to a person, organ, or tissue that contains cells with different genes than the rest of the person, organ, or tissue.

What is a chimera in microbiology?

chimera, in genetics, an organism or tissue that contains at least two different sets of DNA, most often originating from the fusion of as many different zygotes (fertilized eggs). The term is derived from the Chimera of Greek mythology, a fire-breathing monster that was part lion, part goat, and part dragon.

How is chimera formed?

How do you detect chimeric reads?

If you want to detect chimerism in your sequence, the simplest way is to match your gene to NCBI or any other database. Then check the alignment hits. If there chimerism exists, part of your gene will match to different genes. The link is broken.

How do you know if a sequence is chimeric?

Chimeric regions within a query sequence are identified by detecting short sequence fragments that are uncommon within a reference phylogenetic group where the sequence is classified but much more common in another phylogenetic group.

What are the two types of chimera?

Each has a slightly different cause and may result in different symptoms.

  • Microchimerism. In humans, chimerism most commonly occurs when a pregnant woman absorbs a few cells from her fetus.
  • Artificial chimerism.
  • Twin chimerism.
  • Tetragametic chimerism.

What are the benefits of chimeras?

Chimeras are incredibly useful for understanding how animals grow and develop. They might one day be used to grow life-saving organs that can be transplanted into humans.