What is mucin-type O-glycosylation?

What is mucin-type O-glycosylation?

Mucin-type O-glycosylation is a widespread post-translational modification of proteins found in the entire animal kingdom, but also in higher plants. The structural complexity of the chains initiated by O-linked GalNAc exceeds that of N-linked chains by far.

What is O-linked glycosylation?

O-linked glycosylation is the attachment of a sugar molecule to the oxygen atom of serine (Ser) or threonine (Thr) residues in a protein. O-glycosylation is a post-translational modification that occurs after the protein has been synthesised.

What is N and O-linked glycosylation?

N-linked glycosylation requires participation of a special lipid called dolichol phosphate. O-linked glycans attached to the hydroxyl oxygen of serine, threonine, tyrosine, hydroxylysine, or hydroxyproline side-chains, or to oxygens on lipids such as ceramide.

What enzymes are responsible for initiating mucin type glycosylation?

Mucin-type O-glycosylation is unique among various types of glycosylation in that it is initiated by a large family of enzymes (UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts)2 or GalNAc transferases in mammals and PGANTs in Drosophila; EC 2.4. 1.41).

What is the difference between N and O links?

Two common types of glycans, N-linked and O-linked, have been extensively analyzed in the last decades. N-glycans are typically released from glycoproteins by enzymes, while O-glycans are released from glycoproteins by chemical methods.

Why are proteins O-glycosylated?

Steric interactions between carbohydrate and peptide within these clusters induce the peptide core to adopt a stiff and extended conformation and this conformational effect appears to represent a major function of O-glycosylation.

What is O-linked glycosylation quizlet?

O-linked glycosylation is the attachment of a sugar molecule to an oxygen atom in an amino acid residue in a protein.