What is the exonuclease activity?
Terminology: The ability to remove nucleotides one at a time from the end of a chain is called exonuclease activity. (exo = from the exterior or end).
What is proof reading activity?
DNA polymerases are the enzymes that build DNA in cells. During DNA replication (copying), most DNA polymerases can “check their work” with each base that they add. This process is called proofreading. Polymerase uses 3′ to 5′ exonuclease activity to remove the incorrect T from the 3′ end of the new strand.
What is the difference between lagging and leading strand?
1. A leading strand is the strand which is synthesized in the 5′-3’direction while a lagging strand is the strand which is synthesized in the 3′-5′ direction. 2. The leading strand is synthesized continuously while a lagging strand is synthesized in fragments which are called Okazaki fragments.
What is the difference between the leading strand and the lagging strand quizlet?
What is the difference between the leading strand and the lagging strand in DNA replication? The leading strand is synthesized continuously in the 5′ → 3′ direction, while the lagging strand is synthesized discontinuously in the 5′ → 3′ direction.
Is there a leading and lagging strand in PCR?
PCR doesn’t have a leading and lagging strand, nor does it use RNA primers, okazaki fragments, etc. It only replicates continuously (like the leading strand), and uses DNA primers.