What is the surface area of a 6 well plate?

What is the surface area of a 6 well plate?

Useful information for various sizes of cell culture dishes and flasks

Catalog No. Surface area (cm2)
6-well 140675 9.6
12-well 150628 3.5
24-well 142475 1.9

What is the area of a well in a 96 well plate?

96-Well Plates

Cat.No. Brand Growth Area
83-3596 Corning® 0.32cm2
83-3997 Corning® 0.32cm2
83-3598 Corning® 0.32cm2
83-3599 Corning® 0.32cm2

How big is a well in a 6 well plate?

Growth Area of Tissue Culture Plates and Dishes

Growth Area (cm2) Media Volume (ml)
245 mm (square) 500 100-150
6 well 9.5 1.9-2.0
12 well 3.8 0.76-1.14

How many cells are in a well of 96 well plate?

Typically, 20,000 – 75,000 cells are seeded per well in Oris™ and Oris™ Pro 96-well plates, and 2,500 – 10,000 per well in Oris™ Pro 384-well plates.

How much media do I need for a 96-well plate?

For any cell type you can use a minimum of 100ul to a maximum of 200ul culture medium per well in a 96-well plate.

How do you figure surface area?

The total surface area is calculated by adding all the areas on the surface: the areas of the base, top, and lateral surfaces (sides) of the object. This is done using different area formulas and measured in square units. Volume is the amount of space that a three dimensional object takes up.

How much media should be in a 6-well plate?

I usually add 1-2 mL of media in each well in the 6-well plate.

How much does it cost to seed a 6-well plate?

2D cultures

Culture plate format Number of viable cells per well/flask Volume per well/flask
6-well plate 2 x 106 2 ml
12-well plate 0.8 x 106 1 ml
24-well plate 0.48 x 106 0.5 ml
48-well plate 0.16 x 106 0.2 ml

How much volume does a 96-well plate have?

CulturPlate Microplates

Well format 24-well 96-well
Well volume 2.39 mL 400 µL
Recommended working volume 0.5-2.39 mL 80 µL-350 µL
Height (mm) 18.70 14.60
Length (mm) 127.80 127.76

What are the dimensions of a 96-well plate?

The length of the 96-well standard microplate is 127.71 mm x the width 85.43 mm and the height is 14.10 mm (shown in fig….ANSI- /SLAS – Standard 1 to 4 – 2004.

96-Well Standard Plate Dimensions
L 127.71 mm
W 85.43 mm
H 14.10 mm
X1 14.36 mm

How much does it cost to seed a 6 well plate?

How do you incubate a 96-well plate?

Pipette 25 µL protease-inhibitor-supplemented Cell Extraction Buffer into each well. Incubate the plate on ice for 30 minutes. Thoroughly mix the contents of each well by pipetting up and down 5–6 times. A multi-channel pipette is desirable for this application.

How much volume does a 96 well plate hold?

How much can a 96 well plate hold? Well Plate diameter: 6.8 mm; 96 well volume: 360 μL; Working volume: 75-250 μL;

What is the size of a 96-well microplate?

Corning 96-well Microplates Flat Bottom 6.4 0.32 3.2 x 104 360 100 – 200 Round Bottom 6.4 N/A** N/A** 330 100 – 200 V-Bottom 6.4 0.38 3.8 x 104 320 100 – 200 Half Area 4.5 0.16 1.6 x 104 190 50 – 100 Corning 384-well Microplates Standard 2.7 x 2.7* 0.056 5.6 x 103 112 25 – 50

What is the surface area of a Corning well?

**Because these wells are round, the surface area available for cell attachment is dependent on the medium volume used. Corning Multiple Well Plates Single Well Only Well Approx. Total Well Working Diameter Growth Average Volume Volume Plate (Bottom) (mm) Area (cm2) Cell Yield (mL) (mL) 6-well 34.8 9.5 9.5 x 105 16.8 1.9 – 2.9

What are 6 12 24 48 48 and 96 well plates?

6, 12, 24, 48 and 96-Well Plates and Microplates, Corning®. By Corning®. These clear polystyrene plates have nonreversible lids with condensation rings to reduce contamination. Selection of 6-, 12-, 24-, 48 or 96- well plates. They feature individual alphanumerical codes for well identification.