What type of primer is used in PCR?
What would happen if only one primer was used in PCR?
If you only have one primer, you get what is called “asymmetric PCR”. You get just 1 strand made . 1 new copy per cycle. So after 45 cycles you have 45 copies .. not 1,000 copies.
What are the building blocks of new copies of DNA?
DNA is made of chemical building blocks called nucleotides. These building blocks are made of three parts: a phosphate group, a sugar group and one of four types of nitrogen bases. To form a strand of DNA, nucleotides are linked into chains, with the phosphate and sugar groups alternating.
What is the function of primers in a PCR reaction quizlet?
What is the function of the primers in PCR? They polymerize free nucleotides to form the new DNA strands. They provide energy for the DNA polymerization reactions. They provide a 3′ end for the DNA polymerase.
What would happen if polymerase was not added to the PCR reaction?
If you don’t use a hot-start polymerase and keep the reaction for a long period of time, even at 4 degree Celsius dimer formation of your PCR oligonucleotides may occur and the oligonucleotides can be elongated if their 3′-OH end is annealed.