Which of the following types of DNA polymerase has 3 5 exonuclease activity?
Explanation: DNA polymerases I, II, III and IV all has 5’3′ exonuclease activity. DNA polymerases I is the only polymerase to have the 3’5′ exonuclease activity which is the proof reading activity of DNA polymerase.
What is the difference between exonuclease and endonuclease?
The main difference between these enzymes is that endonucleases cleave the phosphodiester bond in the polynucleotide present internal in the polynucleotide chain, whereas exonucleases cleave the phosphodiester bond from the ends.
What can terminal transferase be used for?
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3′ hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT.
What is the function of s1 nuclease?
S1 nuclease is an endonuclease specific for single-stranded DNA or RNA and can be used to study nucleic acid hybridization, mapping RNA start sites and RNA splice sites. This enzyme is five times more active on DNA than RNA, and it will digest all nucleic acids if the enzyme is added to the reaction in excess.
What is the role of DNase I?
Deoxyribonuclease I (DNase I) is an endonuclease which is secreted to cleave DNA in the extracellular space down to an average of tetranucleotides with 5′ monophosphate and 3′ hydroxyl DNA ends (Baranovskii, Buneva, & Nevinsky, 2004). Both single-stranded DNA and double-stranded DNA are degraded by DNase I.
What is the final product of the RNaseH method?
What is the final product of the RNaseH method? Explanation: The final product of the RNaseH method is blunt ended dsDNA. The RNA piece left at the 5′ end is removed by RNase and thus blunt ended dsDNA is left.
What is the substrate of nuclease?
Although its primary substrate is single-stranded, it can also occasionally introduce single-stranded breaks in double-stranded DNA or RNA, or DNA-RNA hybrids. The enzyme hydrolyses single stranded region in duplex DNA such as loops or gaps.
Where is Nucleotidase found?
5′-nucleotidase (EC 3.1. 3.5) is an enzyme which catalyzes the phosphorylytic cleavage of 5’nucleotides. Although originally found in snake venom, the activity of 5’nucleotidase has been described for bacteria and plant cells, and is widely distributed in vertebrate tissue.
Is Nucleotidase present in intestinal juice?
Succus entericus or intestinal juice (pH = 7.8) refers to the secretion of glands of small intestine. It contains many enzymes viz maltase, isomaltase, lipase, lactase, α-dextrinase, enterokinase, aminopeptidase, nucleotidase, nucleosidase, etc. for the digestion of carbohydrates, proteins, fats, nucleic acids etc.
What is the pH of Nucleotidase?
pH-The enzyme has a fairly sharp pH optimum of 7.5, as shown in Fig. 3. The low activity of b nucleotidase at pH 9.4 may explain the separation of this activity from that of 5′-nucleotidase by means of pH, since the latter has a pH optimum of 8.5.