What is the purpose of DNA polymerase III?
The main function of the third polymerase, Pol III, is duplication of the chromosomal DNA, while other DNA polymerases are involved mostly in DNA repair and translesion DNA synthesis. Together with a DNA helicase and a primase, Pol III HE participates in the replicative apparatus that acts at the replication fork.
What is the role of DNA polymerase III?
Summary: DNA polymerase III holoenzyme is the enzyme primarily responsible for replicative DNA synthesis in E. coli. It carries out primer-initiated 5′ to 3′ polymerization of DNA on a single-stranded DNA template, as well as 3′ to 5′ exonucleolytic editing of mispaired nucleotides.
What would happen if ligase was not present?
What would be the consequence(s) for DNA synthesis if DNA ligase were defective? Without DNA ligase activity, Okazaki fragments on the lagging strand would not be joined together; leading strand synthesis would be largely unaffected. Okazaki fragments are found on the strand that replicates discontinuously.
What happens if ligase is mutated?
Primary T-Cell Immunodeficiencies Other features include facial dysmorphia, various degrees of developmental delay, and microcephaly. Hypomorphic mutations in DNA ligase IV can be associated with increased sensitivity to radiotherapy,16 EBV-associated lymphoproliferation and T-cell leukemia.
What is the difference between DNA ligase and polymerase?
DNA ligase works to join the Okazaki fragment during the lagging strand synthesis in semiconservative DNA replication. DNA polymerase catalyzes phosphodiester bond formation between the terminal 3′–OH of the primer and the α-phosphate of the incoming triphosphate.